Lesson BTS:B1-5
Practicing Safety in the Laboratory
 
CD BTS.  Biotechnology Systems
Unit B.  Demonstrate Laboratory Skills as Applied to Biotechnology
Problem Area 1.  Conducting Scientific Investigations in Agriculture
Lesson 5.  Practicing Safety in the Laboratory
 
 

1. What is the chance that an accident will occur?

a. carelessness

b. hazard

c. risk

d. safety

Answer: c

2. What is a device designed to protect the wearer from inhaling harmful dust, liquid droplets, fumes, vapors, and other materials that may be in the air?

a. face shield

b. goggles

c. lung filter

d. respirator

Answer: d

3. How should microbiologically contaminated clothing, equipment, and cultures be sterilized?

a. by autoclave at 121°C for 20 minutes

b. by autoclave at 212°C for 20 minutes

c. by autoclave at 121°F for 20 minutes

d. by autoclave at 212°F for 40 minutes

Answer: a

4. Which of the following is a recommended safety practice?

a. Keep cultures in a sink when not in use.

b. Mix cultures or reagents in such a way as to avoid splashing.

c. When possible, pipet broth cultures by mouth.

d. Wash your hands with cold warm water before leaving the laboratory.

Answer: b
What is safety?
a)It is a danger where risk is present.
b)It is an event that occurs unintentionally.
c)It is preventing injury and loss.
d)It is the chance that an accident will occur.


Answer: c
How is risk defined?
a)It is a danger where risk is present.
b)It is an event that occurs unintentionally.
c)It is preventing injury and loss.
d)It is the chance that an accident will occur.


Answer: d
_____ are devices worn or used to protect from injury.
a)Personal coverage equipment (PCE)
b)Personal occupational equipment (POE)
c)Personal protection equipment (PPE)
d)Personal training equipment (PTE)


Answer: c
Should a serious hazardous situation occur within the laboratory, how should users of the lab react?
a)Call 911 and ask what should be done.
b)Exit calmly and immediately call 911.
c)Immediately wash all exposed skin.
d)Shut and seal all doors and windows.


Answer: b
How often should all work benches and surfaces be decontaminated?
a)after each activity involving microbial cultures
b)before leaving an experiment or at least daily
c)during down time throughout an experiment
d)when the laboratory is first opened for use


Answer: b
Which of the following is an approved practice in the biotechnology laboratory?
a)Carefully lay test-tubes cultures on the bench top when not in use.
b)Carry cultures in a large beaker when moving around the laboratory.
c)Handle all cultures in the laboratory as being potentially pathogenic.
d)Mop up spilled cultures, and then saturate the area with disinfectant.


Answer: c